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215 Liquid Handler/Injector

Single-probe, "workhorse" liquid handler delivers a robust performance for fast and accurate direct injection onto HPLC or LC/MS systems. Suitable for a wide variety of applications with the capability of piercing thick septa. Performs injection, fraction collection and re-injection of collected fractions on one platform.

Features & Benefits
  • Versatile, large-bed liquid handler accommodates more than 330 racks to hold virtually any type of sample vessel


  • Add injection capabilities with the 819 Injection Module; configure with two 819 Injection Modules for increased throughput or for analytical and preparative injections


  • "Make-before-break" stator and large-bore rotor seal prevent pressure spikes from injection at high flows


  • Numerous custom software and rack configurations to meet the demands of your applications


  • New spring-loaded probe is ideal for low- and sub-microliter liquid handling applications, allowing "touch-off" deposition without damaging the substrate or degrading the sample

Applications

  • Analytical analysis and preparative-scale purification of synthetic and biological compounds

  • Semipreparative compound purification and identification as part of Gilson’s LC/MS Autopurification System

  • Multidimensional separations with Solid Phase Extraction (SPE) for the preparation of protein digests for LC/MS/MS analysis

845ZPREP™ System

 

 

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215 Liquid Handler/Injector User's Guide
215 Direct-Inject Liquid Handler/Injector Spec. Sheet
*Automated 2-D HPLC w/Trap Columns for Fractionation, Isolation & Screening of Nat. Products (#227)
*Determination of Reproducibility within an Analytical System via Repeat Injections (#212)
*Novel Approach for Screening of Drug Absorption via an Automated System (#218)
*Optimizing Purified Sample Recovery on Reverse-Phase HPLC Systems (#209)
Manufacturing Standards
  Meets applicable safety and EMC certification standards; UL and CE certified
Arm Speed   >45.7 cm/sec (>18 in./sec) in X dimension
>40.6 cm/sec (>16 in./sec) in Y dimension
Communication Interface   RS-232 or GSIOC; Four inputs (contact closure, TTL, or open-collector), four relay outputs and one switched +24V DC 1A output
Accuracy   Liquid handling: 99.7% with 50 µL of water
Front Panel   8-character display, START and emergency STOP soft keys
Horizontal Motion Strength   X: 5 kg (11.1 lbs.)
Y: 7 kg (15.6 lbs.)
Injection Carryover†   <0.1%
Locator Plate Capacity   Up to five Code 200-Series racks, up to seven Code 20- or 30-Series racks (or a combination of up to five racks of both types), or one Code 500-Series and one Code 200-Series rack
Maximum Syringe Flow Rates for Water  
Syringe Size
(µL)
Max. Flow Rate
(mL/min)
100 3.37
250 8.43
500 16.87
1000 33.75
5000 100
10,000 100
25,000 100
Power Requirements   Frequency: 50–60 Hz
Voltage: 100–120 or 220–240V, mains voltage fluctuations not to exceed ±10% of the nominal voltage
Current rating: 2.0A for 100–120V or 1.0A for 220–240V
Probe Positioning Performance   Accuracy:±0.5 mm in X/Y dimensions±1 mm in Z dimension
Repeatability:±0.25 mm in X/Y/Z dimensions
Probe Rinse   Through a dedicated rinse station for rinsing the inside and outside of the probe; selectable rinse volume and flow rate. Optional inserts for level-sensing, nonleveling-sensing and flow rinse
Sampler Type   X/Y/Z with stationary rack design
Software   Via Gilson UniPoint™ System Software or TRILUTION™ Software
Syringe Capacity   100, 250 or 500 µL; 1, 5, 10 or 25 mL
Syringe Pump   Internal, integral, high-precision, single-piston
Vertical Punch Strength   4.9 kg (11 lbs.)
Reproducibility†   Liquid Handling: 1.37% CV with 50 µL of water

Injection:
<0.9% CV with 1-mL syringe and total loop filling method
Dimensions
(w x d x h)
  91.4 x 61 x 55.8* cm (36 x 24 x 22* in.)
*Maximum height. Z-arm height is adjustable to accommodate vessel heights between 1 and 150 mm (dependent on installed Z-arm).
Weight   39.9 kg (89 lbs.)
† Contact ManSci Customer Service to learn what methods and conditions were used to obtain the values.
 
© 2007 ManSci Inc.